I am new to FISH and I was wondering...Does Affymetrix and Advanced Cell Diagnostics sell the same products with different markings? Are they really the same company? Do they both buy the same oligos from the same oligo supplier and then label differently?
When I reorder the same product from Advanced Cell Diagnostics I get different results. Same is true with the "similar" product from Affymetrix. When I compare the results between Affy and ACD, the results are within a factor of 2 to 3 fold between replicates. The "blobs" are a hard thing to get used to counting and can vary widely within the same cell or tissue.